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Overview This intensive 3-day hands-on cell culture workshop is designed for students, researchers, laboratory technicians, and professionals who want to gain practical experience in mammalian cell culture techniques. The course covers both adherent and non-adherent cell culture systems, guiding participants through the complete workflow from BSL-2 safety principles, aseptic technique, cell thawing, seeding, passaging, counting, viability testing, and troubleshooting. Participants will work directly in the laboratory and practice essential techniques required for independent cell culture work. The workshop combines theoretical background with practical training to help participants build confidence in sterile handling, experimental planning, and data interpretation. By the end of the course, participants will have a strong foundation in routine mammalian cell culture and will be able to perform basic cell culture workflows safely and independently.   Learning Objectives By the end of this workshop, participants will be able to: Understand basic principles of mammalian cell culture Apply BSL-2 laboratory safety guidelines Perform aseptic work under a biosafety cabinet Handle both adherent and non-adherent cells Thaw, seed, and maintain mammalian cells Perform cell passaging using appropriate techniques Count cells manually and understand automated cell counting principles Assess cell viability using Trypan Blue and MTT-based methods Recognize common contamination risks and technical problems Troubleshoot basic cell culture issues Interpret simple cell culture results and document experiments properly   Target Audience This course is ideal for: Master’s and PhD students Research assistants and laboratory technicians Early-career researchers Biotechnology and biomedical science students Professionals entering cell culture-based laboratory work Scientists who want to refresh or strengthen their basic cell culture skills Participants preparing for laboratory work in academia, biotech, or pharmaceutical research   Course Program Day 1: BSL-2 Safety, Aseptic Technique & Seeding Fundamentals Time  Activity  Format  09:00 – 09:30  Introduction & Course Overview  Lecture  09:30 – 10:30  Introduction to BSL-2 Guidelines and Laboratory Safety  Lecture  10:30 – 10:45  Coffee Break    10:45 – 12:00  principles of Mammalian Cell Culture: Adherent vs. Non-Adherent Cells  Lecture  12:00 – 12:30  Introduction to Aseptic Technique and Biosafety Cabinet Work  Demonstration  12:30 – 13:30  Lunch Break    13:30 – 15:00  Cell Thawing and Recovery: Preparing Cells for Culture  Laboratory  15:00 – 15:15  Coffee Break    15:15 – 16:30  Cell Seeding Practice: Adherent and Non-Adherent Cell Systems  Laboratory  16:30 – 17:00  Experimental Documentation, Discussion & Preparation for Day 2  Discussion & Q&A    Day 2: Passaging, Cell Counting & Viability Testing Time  Activity  Format  09:00 – 09:20  Welcome, Recap of Day 1 and Questions  Q&A  09:20 – 10:30  Principles of Cell Passaging and Culture Maintenance  Lecture  10:30 – 10:45  Coffee Break    10:45 – 12:00  Passaging of Adherent Cells: Trypsinization and Re-Seeding  Laboratory  12:00 – 12:30  Handling and Maintenance of Non-Adherent Cells  Laboratory  12:30 – 13:30  Lunch Break    13:30 – 14:30  Manual Cell Counting Using Hemocytometer  Laboratory  14:30 – 15:15  Introduction to Automated Cell Counting and Comparison of Methods  Demonstration  15:15 – 15:30  Coffee Break    15:30 – 16:30  Cell Viability Assessment Using Trypan Blue  Laboratory  16:30 – 17:00  Data Recording, Calculation Practice & Troubleshooting Discussion  Discussion & Q&A    Day 3: Hood Mastery, Viability Assays, Troubleshooting & Interpretation Time  Activity  Format  09:00 – 09:20  Welcome, Recap of Day 2 and Questions  Q&A  09:20 – 10:30  Hood Technique Refinement: Best Practices for Sterile Work  Laboratory  10:30 – 10:45  Coffee Break    10:45 – 12:00  Common Cell Culture Problems: Contamination, Poor Growth, Cell Death and Handling Errors  Lecture & Discussion  12:00 – 12:30  Introduction to MTT Assay and Cell Viability Readouts  Lecture  12:30 – 13:30  Lunch Break    13:30 – 14:45  MTT Assay Workflow or Demonstration-Based Viability Analysis  Laboratory / Demonstration  14:45 – 15:30  Data Interpretation Workshop: Understanding Cell Growth and Viability Results  Workshop  15:30 – 15:45  Coffee Break    15:45 – 16:30  Troubleshooting Case Studies and Group Discussion  Discussion  16:30 – 17:00  Final Quiz, Feedback Session and Certificate Information  Q&A    What Participants Will Gain Participants will gain: Hands-on experience in mammalian cell culture workflows Practical laboratory skills in sterile and aseptic handling Confidence working under a biosafety cabinet Experience with adherent and non-adherent cell systems Practical knowledge of thawing, seeding, passaging, and maintaining cells Experience with manual cell counting and viability assessment Understanding of Trypan Blue and MTT-based viability assays Troubleshooting strategies for common cell culture problems Better understanding of cell culture documentation and data interpretation Preparation for independent laboratory work in academic or industrial settings   XTech Academy in collaboration with Springer Campus Location: XTech Academy, Heidelberg Duration: 4 days Format: Hands-on training course Level: Intermediate  

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Overview This intensive 3-day hands-on training course provides participants with a practical and structured introduction to PCR, quantitative real-time PCR (qPCR), and gene expression analysis. The course covers the full workflow from experimental design, primer design, RNA/DNA extraction, RNA quality control, cDNA synthesis, qPCR setup, amplification monitoring, data analysis, normalization strategies, and biological interpretation. Participants will gain both theoretical understanding and practical experience in key molecular biology techniques used in research, diagnostics, biotechnology, and biomedical applications. The workshop combines lectures, demonstrations, hands-on laboratory sessions, computer-based analysis, and troubleshooting discussions. By the end of the course, participants will understand how PCR and qPCR experiments are designed, performed, analyzed, and interpreted, with a strong focus on reliable experimental planning and meaningful gene expression analysis.   Learning Objectives By the end of this workshop, participants will be able to: Understand the basic principles of conventional PCR and real-time qPCR Explain the differences between PCR and qPCR workflows Understand key considerations in experimental design Apply basic primer design strategies for PCR and qPCR experiments Work according to BSL-2 laboratory safety guidelines Perform sample preparation and RNA/DNA extraction workflows Assess RNA quality and quantity before downstream applications Understand the principles of reverse transcription and cDNA synthesis Prepare qPCR reactions with appropriate reagents and controls Understand amplification curves and Ct values Apply the ΔΔCt method for relative gene expression analysis Use normalization strategies for reliable data interpretation Visualize gene expression results and understand basic statistical considerations Identify common technical issues in PCR and qPCR experiments Apply troubleshooting strategies and best practices   Target Audience This course is ideal for: Master’s and PhD students Research assistants and laboratory technicians Biomedical, biotechnology, molecular biology, and life science students Researchers entering molecular biology or gene expression analysis Professionals working in academic, diagnostic, biotech, or pharmaceutical laboratories Scientists who want to strengthen their PCR, qPCR, and data interpretation skills Participants interested in learning practical gene expression workflows from sample preparation to analysis   Course Program Day 1: Fundamentals of PCR & Experimental Design Focus: Principles, primer design and workflow setup Time  Activity  Format  09:00 – 09:30  Welcome & Course Overview  Lecture  09:30 – 10:30  Introduction to BSL-2 Guidelines & Laboratory Safety  Lecture  10:30 – 11:15  PCR Principles: Conventional PCR vs qPCR  Lecture  11:15 – 11:30  Coffee Break    11:30 – 12:30  Experimental Design & Primer Design Strategies  Lecture + Demo  12:30 – 13:30  Lunch Break    13:30 – 15:00  Sample Preparation & RNA/DNA Extraction  Laboratory  15:00 – 15:15  Coffee Break    15:15 – 16:30  RNA Quality Control & Quantification  Laboratory  16:30 – 17:00  Q&A & Wrap-up  Discussion & Q&A    Day 2: cDNA Synthesis & qPCR Workflow Focus: Reverse transcription and amplification Time  Activity  Format  09:00 – 09:30  Overview Day 3  Lecture  09:30 – 10:30  Ct Values, ΔΔCt Method & Normalization Strategies  Lecture  10:30 – 10:45  Coffee Break    10:45 – 12:30  qPCR Data Analysis & Interpretation  Computer / Laboratory  12:30 – 13:30  Lunch Break    13:30 – 14:30  Gene Expression Visualization & Statistics  Lecture + Demo  14:30 – 15:30  Experimental Troubleshooting & Best Practices  Discussion  15:30 – 16:00  Mini Quiz / Case Study Discussion  Discussion  16:00 – 16:30  Final Wrap-up, Feedback & Certificates  Q&A    Day 3: Gene Expression Analysis & Data Interpretation Focus: Data analysis and biological interpretation Time  Activity  Format  09:00 – 09:30  Overview Day 3  Lecture  09:30 – 10:30  Ct Values, ΔΔCt Method & Normalization Strategies  Lecture  10:30 – 10:45  Coffee Break    10:45 – 12:30  qPCR Data Analysis & Interpretation  Computer / Laboratory  12:30 – 13:30  Lunch Break    13:30 – 14:30  Gene Expression Visualization & Statistics  Lecture + Demo  14:30 – 15:30  Experimental Troubleshooting & Best Practices  Discussion  15:30 – 16:00  Mini Quiz / Case Study Discussion  Discussion  16:00 – 16:30  Final Wrap-up, Feedback & Certificates  Q&A    What Participants Will Gain Participants will gain: Practical understanding of PCR and qPCR workflows Hands-on experience with sample preparation and RNA/DNA extraction Practical knowledge of RNA quality control and quantification Understanding of primer design and experimental planning Experience with cDNA synthesis and reverse transcription workflows Practical insight into qPCR setup, controls, and optimization Understanding of amplification curves, Ct values, and qPCR readouts Skills in gene expression analysis using the ΔΔCt method Knowledge of normalization strategies and basic statistical interpretation Experience with data visualization and biological interpretation Troubleshooting strategies for common PCR and qPCR problems Preparation for independent work in molecular biology, diagnostics, biotechnology, and gene expression research  

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