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€ 3.900

(excluding 19% VAT)
Course Registration

CAR-T Cell Design & Engineering | 4-Day Hands-on Training Program

Practical Training in CAR-T Cell Therapy, T Cell Engineering & Translational Applications

Course Format

Course Format

Additional information

Duration

4 Days
Training Type

Hands-on Laboratory and Project-Based Workshop
Location

XTech Academy, Heidelberg, Germany
Class Size

Small groups to ensure intensive supervision and individualized support
Level

Intermediate
Language

English or German, depending on the selected course date
Certificate

Participants receive an XTech Academy Certificate of Completion upon successful participation

Course Format

Course Details

Overview

This intensive 4-day hands-on training program introduces participants to the principles and experimental workflow of CAR-T cell design and engineering.

The course covers key concepts in immunotherapy, CAR structure, antigen recognition, signaling domains, target antigen selection, vector systems, gene delivery, T cell isolation, T cell activation, transduction/transfection setup, CAR-T cell expansion, viability assessment, flow cytometry-based CAR-T analysis, cytotoxicity assays, and translational applications.

Participants will gain practical insight into how CAR-T cell strategies are designed, generated, characterized, and evaluated in research and translational settings.

 

Learning Objectives

By the end of this workshop, participants will be able to:

  • Understand the principles of immunotherapy and CAR-T cell engineering
  • Apply BSL-2 laboratory safety guidelines
  • Explain CAR structure, antigen recognition, and signaling domains
  • Understand target antigen selection and CAR design strategies
  • Compare viral and non-viral gene delivery approaches
  • Understand T cell biology, activation, and expansion
  • Perform or observe T cell isolation and activation workflows
  • Understand transduction/transfection setup for CAR introduction
  • Assess CAR-T cell viability and expansion
  • Understand flow cytometry principles for CAR-T characterization
  • Analyze surface markers and CAR expression
  • Understand cytotoxicity assays and functional readouts
  • Discuss challenges such as toxicity, resistance, and tumor escape
  • Design a basic CAR-T strategy for a selected cancer target

 

Target Audience

This course is ideal for:

  • Master’s and PhD students
  • Research assistants and laboratory technicians
  • Immunology, oncology, cell therapy, and biotechnology researchers
  • Scientists interested in CAR-T and immune cell engineering
  • Professionals working in translational research, biotech, or pharmaceutical development
  • Participants interested in cancer immunotherapy and next-generation cell therapies

 

 

Course Program

Day 1: Introduction to CAR-T Cell Therapy & Experimental Design 

Focus: Fundamentals of CAR-T technology 
Time  Activity  Format 
09:00 – 09:30  Welcome & Course Overview  Lecture 
09:30 – 10:30  Introduction to BSL-2 Guidelines & Laboratory Safety  Lecture 
10:30 – 11:15  Principles of Immunotherapy & CAR-T Cell Engineering  Lecture 
11:15 – 11:30  Coffee Break   
11:30 – 12:30  CAR Structure, Antigen Recognition & Signaling Domains  Lecture 
12:30 – 13:30  Lunch Break   
13:30 – 15:00  Target Antigen Selection & CAR Design Strategies  Lecture + Discussion 
15:00 – 15:15  Coffee Break   
15:15 – 16:30  Introduction to Vector Systems & Gene Delivery Methods  Lecture 
16:30 – 17:00  Q&A & Wrap-up  Discussion & Q&A 

Day 2: T Cell Isolation, Activation & Gene Delivery

Focus: CAR introduction into T cells
Time  Activity  Format 
09:00 – 09:30  Overview Day 2 & Experimental Planning  Q&A 
09:30 – 10:30  T Cell Biology & Activation Principles  Lecture 
10:30 – 10:45  Coffee Break   
10:45 – 12:30  Hands-on: T Cell Isolation & Activation Workflow  Lab 
12:30 – 13:30  Lunch Break   
13:30 – 15:00  Viral & Non-viral Gene Delivery Approaches  Lecture + Demo 
15:00 – 15:15  Coffee Break   
15:15 – 16:30  Hands-on: Transduction / Transfection Setup  Lab 
16:30 – 17:00  Wrap-up & Discussion  Discussion 

Day 3: CAR-T Cell Expansion & Functional Characterization

Focus: Evaluating engineered CAR-T cells
Time  Activity  Format 
09:00 – 09:30  Overview Day 3  Lecture 
09:30 – 10:30  Expansion & Maintenance of CAR-T Cells  Lecture 
10:30 – 10:45  Coffee Break   
10:45 – 12:30  Hands-on: Cell Expansion & Viability Assessment  Lab 
12:30 – 13:30  Lunch Break   
13:30 – 14:30  Flow Cytometry Principles for CAR-T Analysis  Lecture 
14:30 – 15:30  Hands-on: Surface Marker & CAR Expression Analysis  Lab 
15:30 – 15:45  Coffee Break   
15:45 – 16:30  Cytotoxicity Assays & Functional Readouts  Lecture + Demo 
16:30 – 17:00  Wrap-up  Discussion 

Day 4: Advanced CAR-T Applications & Translational Perspectives

Focus: Clinical translation and next-generation CAR-T
Time  Activity  Format 
09:00 – 10:00  Challenges in CAR-T Therapy: Toxicity, Resistance & Tumor Escape  Lecture 
10:00 – 10:15  Coffee Break   
10:15 – 11:30  Next-Generation CAR-T Technologies & Solid Tumor Applications  Lecture 
11:30 – 12:30  Combination Therapies & Genome Editing in CAR-T Engineering  Lecture 
12:30 – 13:30  Lunch Break   
13:30 – 15:00  Mini Project: Design a CAR-T Strategy for a Selected Cancer Target  Group Work 
15:00 – 15:15  Coffee Break   
15:15 – 16:00  Group Presentations & Scientific Discussion  Presentation & Discussion 
16:00 – 16:30  Final Wrap-up, Feedback & Certificates  Q&A 

What Participants Will Gain

Participants will gain:

  • Understanding of CAR-T cell therapy principles
  • Practical insight into CAR design and antigen selection
  • Knowledge of vector systems and gene delivery strategies
  • Experience with T cell isolation and activation workflows
  • Understanding of CAR-T expansion and viability assessment
  • Insight into flow cytometry-based CAR-T characterization
  • Knowledge of cytotoxicity assays and functional readouts
  • Understanding of next-generation CAR-T technologies
  • Experience designing a CAR-T strategy in a mini project
  • Preparation for future work in immunotherapy and cell therapy research

 

Course Details

  • Location: XTech Academy, Heidelberg
  • Duration: 4 days
  • Format: Hands-on laboratory and project-based training course
  • Level: Intermediate
  • Price: €3,900 excluding 19% VAT
  • Focus: CAR-T therapy, immunotherapy, CAR design, T cell activation, gene delivery, CAR-T expansion, flow cytometry, cytotoxicity assays, translational applications, and next-generation CAR-T technologies

 

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