0

€ 2.400

(excluding 19% VAT)
Course Registration

3-Day Intensive Cell Culture Training

Course Format

Course Format

Additional information

In German

08 – 10 December 2025
09 – 11 February 2026
08 – 10 June 2026
12 – 14 October 2026
In English

20 – 22 October 2026
13 – 15 April 2026
14 – 16 September 2026
07 – 09 December 2026

Course Format

Course Details

Overview

This intensive 3-day hands-on cell culture workshop is designed for students, researchers, laboratory technicians, and professionals who want to gain practical experience in mammalian cell culture techniques.

The course covers both adherent and non-adherent cell culture systems, guiding participants through the complete workflow from BSL-2 safety principles, aseptic technique, cell thawing, seeding, passaging, counting, viability testing, and troubleshooting.

Participants will work directly in the laboratory and practice essential techniques required for independent cell culture work. The workshop combines theoretical background with practical training to help participants build confidence in sterile handling, experimental planning, and data interpretation.

By the end of the course, participants will have a strong foundation in routine mammalian cell culture and will be able to perform basic cell culture workflows safely and independently.

 

Learning Objectives

By the end of this workshop, participants will be able to:

  • Understand basic principles of mammalian cell culture
  • Apply BSL-2 laboratory safety guidelines
  • Perform aseptic work under a biosafety cabinet
  • Handle both adherent and non-adherent cells
  • Thaw, seed, and maintain mammalian cells
  • Perform cell passaging using appropriate techniques
  • Count cells manually and understand automated cell counting principles
  • Assess cell viability using Trypan Blue and MTT-based methods
  • Recognize common contamination risks and technical problems
  • Troubleshoot basic cell culture issues
  • Interpret simple cell culture results and document experiments properly

 

Target Audience

This course is ideal for:

  • Master’s and PhD students
  • Research assistants and laboratory technicians
  • Early-career researchers
  • Biotechnology and biomedical science students
  • Professionals entering cell culture-based laboratory work
  • Scientists who want to refresh or strengthen their basic cell culture skills
  • Participants preparing for laboratory work in academia, biotech, or pharmaceutical research

 

Course Program

Day 1: BSL-2 Safety, Aseptic Technique & Seeding Fundamentals

Time  Activity  Format 
09:00 – 09:30  Introduction & Course Overview  Lecture 
09:30 – 10:30  Introduction to BSL-2 Guidelines and Laboratory Safety  Lecture 
10:30 – 10:45  Coffee Break   
10:45 – 12:00  principles of Mammalian Cell Culture: Adherent vs. Non-Adherent Cells  Lecture 
12:00 – 12:30  Introduction to Aseptic Technique and Biosafety Cabinet Work  Demonstration 
12:30 – 13:30  Lunch Break   
13:30 – 15:00  Cell Thawing and Recovery: Preparing Cells for Culture  Laboratory 
15:00 – 15:15  Coffee Break   
15:15 – 16:30  Cell Seeding Practice: Adherent and Non-Adherent Cell Systems  Laboratory 
16:30 – 17:00  Experimental Documentation, Discussion & Preparation for Day 2  Discussion & Q&A 

 

Day 2: Passaging, Cell Counting & Viability Testing

Time  Activity  Format 
09:00 – 09:20  Welcome, Recap of Day 1 and Questions  Q&A 
09:20 – 10:30  Principles of Cell Passaging and Culture Maintenance  Lecture 
10:30 – 10:45  Coffee Break   
10:45 – 12:00  Passaging of Adherent Cells: Trypsinization and Re-Seeding  Laboratory 
12:00 – 12:30  Handling and Maintenance of Non-Adherent Cells  Laboratory 
12:30 – 13:30  Lunch Break   
13:30 – 14:30  Manual Cell Counting Using Hemocytometer  Laboratory 
14:30 – 15:15  Introduction to Automated Cell Counting and Comparison of Methods  Demonstration 
15:15 – 15:30  Coffee Break   
15:30 – 16:30  Cell Viability Assessment Using Trypan Blue  Laboratory 
16:30 – 17:00  Data Recording, Calculation Practice & Troubleshooting Discussion  Discussion & Q&A 

 

Day 3: Hood Mastery, Viability Assays, Troubleshooting & Interpretation

Time  Activity  Format 
09:00 – 09:20  Welcome, Recap of Day 2 and Questions  Q&A 
09:20 – 10:30  Hood Technique Refinement: Best Practices for Sterile Work  Laboratory 
10:30 – 10:45  Coffee Break   
10:45 – 12:00  Common Cell Culture Problems: Contamination, Poor Growth, Cell Death and Handling Errors  Lecture & Discussion 
12:00 – 12:30  Introduction to MTT Assay and Cell Viability Readouts  Lecture 
12:30 – 13:30  Lunch Break   
13:30 – 14:45  MTT Assay Workflow or Demonstration-Based Viability Analysis  Laboratory / Demonstration 
14:45 – 15:30  Data Interpretation Workshop: Understanding Cell Growth and Viability Results  Workshop 
15:30 – 15:45  Coffee Break   
15:45 – 16:30  Troubleshooting Case Studies and Group Discussion  Discussion 
16:30 – 17:00  Final Quiz, Feedback Session and Certificate Information  Q&A 

 

What Participants Will Gain

Participants will gain:

  • Hands-on experience in mammalian cell culture workflows
  • Practical laboratory skills in sterile and aseptic handling
  • Confidence working under a biosafety cabinet
  • Experience with adherent and non-adherent cell systems
  • Practical knowledge of thawing, seeding, passaging, and maintaining cells
  • Experience with manual cell counting and viability assessment
  • Understanding of Trypan Blue and MTT-based viability assays
  • Troubleshooting strategies for common cell culture problems
  • Better understanding of cell culture documentation and data interpretation
  • Preparation for independent laboratory work in academic or industrial settings

 

XTech Academy in collaboration with Springer Campus

  • Location: XTech Academy, Heidelberg
  • Duration: 4 days
  • Format: Hands-on training course
  • Level: Intermediate

 

Reviews

There are no reviews yet.

Be the first to review “3-Day Intensive Cell Culture Training”

Your email address will not be published. Required fields are marked *