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4-Day Hands-On CRISPR-Cas9 Training Program

Practical Training in Genome Editing, Molecular Biology & Data Interpretation

Course Format

Course Format

Additional information

In German

15 – 18 December 2025
16 – 19 February 2026
15 – 18 June 2026
19 – 22 October 2026
In English

27 – 30 October 2026
20 – 23 April 2026
21 – 24 September 2026
14 – 17 December 2026

Course Format

Course Format

Course Details

Overview

This intensive 4-day hands-on CRISPR-Cas9 training program introduces participants to the principles, design strategy, and laboratory workflow of CRISPR-Cas9 genome editing.

The course covers the complete workflow from CRISPR-Cas9 theory and target selection to gRNA design, reagent preparation, transfection methods, DNA extraction, PCR amplification, gel electrophoresis, data interpretation, and troubleshooting.

Participants will gain both theoretical understanding and practical laboratory experience in genome editing-related techniques. The workshop combines guided lectures, demonstrations, and hands-on laboratory sessions to help participants understand how CRISPR-Cas9 experiments are planned, performed, analyzed, and interpreted.

By the end of the course, participants will have a strong foundation in CRISPR-Cas9 workflows and will be able to understand the key experimental steps required for successful genome editing projects.

 

Learning Objectives

By the end of this workshop, participants will be able to:

  • Understand the basic principles and mechanism of CRISPR-Cas9 genome editing
  • Explain the role of Cas9, gRNA, PAM sequence, and target DNA
  • Understand how CRISPR-Cas9 experiments are designed
  • Select suitable target regions and understand basic gRNA design principles
  • Apply BSL-2 laboratory safety guidelines in genome editing-related workflows
  • Understand reagent preparation steps for CRISPR-Cas9 experiments
  • Become familiar with transfection methods and their applications
  • Perform or observe key molecular biology techniques including DNA extraction and PCR
  • Understand the role of gel electrophoresis in CRISPR workflow analysis
  • Interpret basic experimental outcomes from CRISPR-related assays
  • Recognize common technical challenges in CRISPR-Cas9 experiments
  • Apply troubleshooting strategies for experimental planning and analysis

 

Target Audience

This course is ideal for:

  • Master’s and PhD students
  • Research assistants and laboratory technicians
  • Biotechnology and biomedical science students
  • Researchers new to genome editing technologies
  • Scientists working in molecular biology, cell biology, genetics, or biotechnology
  • Professionals who want to gain practical insight into CRISPR-Cas9 workflows
  • Participants preparing for laboratory work involving gene editing, PCR, DNA analysis, or transfection methods

 

Course Program

Day 1: Introduction to CRISPR-Cas9 & Design Principles

Time  Activity  Format 
09:00 – 09:30  Introduction & Course Overview  Lecture 
09:30 – 11:00  Introduction to BSL-2 Guidelines and Laboratory Safety  Lecture 
11:00 – 11:15  Coffee Break   
11:15 – 12:30  CRISPR-Cas9 Theory: Background, Mechanism and Applications  Lecture 
12:30 – 13:30  Lunch Break   
13:30 – 15:00  CRISPR-Cas9 Target Selection and Experimental Design Principles  Lecture / Workshop 
15:00 – 15:15  Coffee Break   
15:15 – 16:30  Introduction to gRNA Design Tools and Target Evaluation  Workshop 
16:30 – 17:00  Group Discussion, Questions and Preparation for Day 2  Discussion & Q&A 

 

Day 2: Reagent Preparation & Transfection Methods

Time  Activity  Format 
09:00 – 09:20  Welcome, Recap of Day 1 and Questions  Q&A 
09:20 – 10:30  CRISPR-Cas9 Reagent Preparation: Key Components and Workflow Planning  Lecture / Demonstration 
10:30 – 10:45  Coffee Break   
10:45 – 12:00  CRISPR-Cas9 Reagent Preparation Practice or Demonstration  Laboratory 
12:00 – 13:00  Lunch Break   
13:00 – 14:00  Introduction to Transfection Methods and Experimental Considerations  Lecture 
14:00 – 15:30  CRISPR-Cas9 Transfection Workflow: Setup and Practical Application  Laboratory 
15:30 – 15:45  Coffee Break   
15:45 – 16:30  Factors Affecting Transfection Efficiency and Cell Viability  Discussion 
16:30 – 17:00  Documentation, Troubleshooting Discussion and Preparation for Day 3  Discussion & Q&A 

 

Day 3: DNA Handling, PCR & Gel Electrophoresis

Time  Activity  Format 
09:00 – 09:20  Welcome, Recap of Day 2 and Questions  Q&A 
09:20 – 10:30  DNA Extraction and PCR: Theory and Workflow Overview  Lecture 
10:30 – 11:00  Coffee Break   
11:00 – 12:00  DNA Extraction from Cell Samples  Laboratory 
12:00 – 13:00  Lunch Break   
13:00 – 14:30  PCR Setup, Amplification Strategy and Sample Preparation  Laboratory 
14:30 – 15:15  Introduction to Agarose Gel Electrophoresis  Lecture / Demonstration 
15:15 – 15:30  Coffee Break   
15:30 – 16:30  Gel Loading, Electrophoresis and Visualization of PCR Products  Laboratory 
16:30 – 17:00  Result Documentation and Discussion  Discussion & Q&A 

 

Day 4: Data Analysis, Troubleshooting & Scientific Interpretation

Time  Activity  Format 
09:00 – 09:20  Welcome, Recap of Day 3 and Questions  Q&A 
09:20 – 10:30  Introduction to CRISPR-Cas9 Outcome Analysis  Lecture 
10:30 – 10:45  Coffee Break   
10:45 – 12:00  Data Interpretation: PCR and Gel Electrophoresis Results  Workshop 
12:00 – 13:00  Lunch Break   
13:00 – 14:00  Common Challenges in CRISPR-Cas9 Experiments  Lecture & Discussion 
14:00 – 15:00  Troubleshooting Case Studies: Design, Transfection, PCR and Analysis  Workshop 
15:00 – 15:30  Group Discussion and Experimental Planning Reflection  Discussion 
15:30 – 16:00  Final Quiz, Feedback Session and Certificate Information  Q&A 

 

What Participants Will Gain

Participants will gain:

  • Practical understanding of CRISPR-Cas9 genome editing workflows
  • Knowledge of CRISPR-Cas9 theory, mechanism, and experimental design
  • Experience with target selection and gRNA design principles
  • Understanding of BSL-2 safety and sterile workflow requirements
  • Insight into CRISPR-Cas9 reagent preparation
  • Practical experience with transfection workflow concepts
  • Hands-on or demonstration-based experience with DNA extraction
  • Practical knowledge of PCR setup and amplification
  • Understanding of gel electrophoresis and result visualization
  • Basic skills in CRISPR-related data interpretation
  • Troubleshooting strategies for common CRISPR-Cas9 experimental issues
  • Preparation for future work in genome editing, molecular biology, and biotechnology research

 

XTech Academy in collaboration with Springer Campus

  • Location: XTech Academy, Heidelberg
  • Duration: 4 days
  • Format: Hands-on training course
  • Level: Intermediate
  • Workshop Schedule

 

1 review for 4-Day Hands-On CRISPR-Cas9 Training Program

  1. Felix Dorn –

    I participated in a 4-day hands-on CRISPR course, which was very well organized both in terms of timing and content. Before the training, participants received materials to get familiar with the topic, which was very helpful. Each module included a theoretical introduction, followed by a hands-on demonstration and a practical session to apply what we had learned.

    The instructors were very friendly, supportive, and took the time to address individual questions and challenges. It’s also great that participants can reach out to XTechAcademy even after the course for further questions or support.

    The laboratory was modern and well-equipped, providing an excellent environment for learning. The building also offered a comfortable lounge area where participants could freely enjoy drinks, fruit, and even prepare something to eat in between sessions.

    Overall, it was a great learning experience and an excellent opportunity to deepen practical skills in CRISPR technology.

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