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€ 3.400

(excluding 19% VAT)
Course Registration

Flow Cytometry (FACS) | 3-Day Hands-on Training Program

Practical Training in Flow Cytometry, Cell Staining, Data Acquisition & Analysis

Course Format

Course Format

Additional information

Duration

3 Days
Training Type

Hands-on Laboratory and Computer-Based Workshop
Location

XTech Academy, Heidelberg, Germany
Class Size

Small groups to ensure intensive supervision and individualized support
Level

Beginner to Intermediate
Language

English or German, depending on the selected course date
Certificate

Participants receive an XTech Academy Certificate of Completion upon successful participation

Course Format

Course Details

Overview

This intensive 3-day hands-on training program provides participants with a practical introduction to flow cytometry and fluorescence-activated cell analysis.

The course covers the full workflow from flow cytometry principles, FSC/SSC interpretation, fluorescence detection, fluorophore and antibody selection, panel design, sample preparation, surface and intracellular staining, compensation, controls, data acquisition, gating strategies, and data interpretation.

Participants will gain both theoretical understanding and practical experience with flow cytometry workflows used in immunology, cancer biology, stem cell research, and biomedical laboratories.

 

Learning Objectives

By the end of this workshop, participants will be able to:

  • Understand the principles of flow cytometry and fluorescence detection
  • Explain FSC, SSC, and fluorescence signal interpretation
  • Apply BSL-2 laboratory safety guidelines
  • Understand fluorophores, antibodies, and panel design basics
  • Prepare samples for flow cytometry analysis
  • Perform surface marker staining and controls
  • Understand compensation and gating strategies
  • Acquire data using a flow cytometer
  • Understand intracellular staining and fixation/permeabilization workflows
  • Analyze FACS data using software tools
  • Interpret cell populations and quantitative readouts
  • Troubleshoot common flow cytometry problems
  • Understand applications in immunology, cancer, and stem cell research

 

Target Audience

This course is ideal for:

  • Master’s and PhD students
  • Research assistants and laboratory technicians
  • Immunology, cancer biology, stem cell, and biomedical researchers
  • Biotechnology and life science students
  • Professionals entering flow cytometry-based research
  • Scientists who want to strengthen practical FACS skills

 

 

Course Program

Day 1: Fundamentals of Flow Cytometry & Experimental Design

Focus: Principles, instrumentation and sample preparation
Time  Activity  Format 
09:00 – 09:30  Welcome & Course Overview  Lecture 
09:30 – 10:30  Introduction to BSL-2 Guidelines & Laboratory Safety  Lecture 
10:30 – 11:15  Principles of Flow Cytometry: FSC, SSC & Fluorescence Detection  Lecture 
11:15 – 11:30  Coffee Break   
11:30 – 12:30  Fluorophores, Antibodies & Panel Design Basics  Lecture 
12:30 – 13:30  Lunch Break   
13:30 – 15:00  Sample Preparation & Cell Staining Workflow  Lab 
15:00 – 15:15  Coffee Break   
15:15 – 16:30  Hands-on: Surface Marker Staining & Controls  Lab 
16:30 – 17:00  Q&A & Wrap-up  Discussion & Q&A 

 

Day 2: Data Acquisition & Advanced Staining Strategies

Focus: Running samples and multicolor analysis
Time  Activity  Format 
09:00 – 09:30  Overview Day 2 & Instrument Setup  Q&A 
09:30 – 10:30  Compensation, Controls & Gating Strategies  Lecture 
10:30 – 10:45  Coffee Break   
10:45 – 12:30  Hands-on: Data Acquisition on Flow Cytometer  Lab 
12:30 – 13:30  Lunch Break   
13:30 – 14:30  Intracellular Staining & Fixation/Permeabilization Principles  Lecture + Demo 
14:30 – 15:30  Hands-on: Intracellular / Apoptosis Staining  Lab 
15:30 – 15:45  Coffee Break   
15:45 – 16:30  Troubleshooting Flow Cytometry Experiments  Discussion 
16:30 – 17:00  Wrap-up  Discussion & Q&A 

 

Day 3: Data Analysis, Interpretation & Applications

Focus: Analysis and biological interpretation
Time  Activity  Format 
09:00 – 09:30  Introduction to FACS Data Analysis Software  Lecture + Demo 
09:30 – 11:00  Hands-on: Gating & Population Analysis  Computer Session 
11:00 – 11:15  Coffee Break   
11:15 – 12:30  Quantitative Analysis & Data Interpretation  Computer Session 
12:30 – 13:30  Lunch Break   
13:30 – 14:30  Applications of Flow Cytometry in Immunology, Cancer & Stem Cell Research  Lecture 
14:30 – 15:15  Experimental Design for Multicolor Panels  Discussion 
15:15 – 15:30  Coffee Break   
15:30 – 16:00  Case Study Discussion & Presentation of Results  Discussion 
16:00 – 16:30  Final Wrap-up, Feedback & Certificates  Q&A 

 

 

What Participants Will Gain

Participants will gain:

  • Practical understanding of flow cytometry workflows
  • Experience with sample preparation and cell staining
  • Knowledge of surface marker and intracellular staining strategies
  • Understanding of compensation, controls, and gating
  • Practical insight into data acquisition and analysis
  • Skills in population analysis and biological interpretation
  • Troubleshooting strategies for common FACS issues
  • Preparation for flow cytometry applications in research and industry

 

Course Details

  • Location: XTech Academy, Heidelberg
  • Duration: 3 days
  • Format: Hands-on laboratory and computer-based training course
  • Level: Beginner to intermediate
  • Price: €3,400 excluding 19% VAT
  • Focus: Flow cytometry, FACS, fluorophores, antibody panels, cell staining, compensation, gating, data acquisition, data analysis, and troubleshooting

 

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