Course Details
Overview
This intensive 4-day hands-on CRISPR-Cas9 training program introduces participants to the principles, design strategy, and laboratory workflow of CRISPR-Cas9 genome editing.
The course covers the complete workflow from CRISPR-Cas9 theory and target selection to gRNA design, reagent preparation, transfection methods, DNA extraction, PCR amplification, gel electrophoresis, data interpretation, and troubleshooting.
Participants will gain both theoretical understanding and practical laboratory experience in genome editing-related techniques. The workshop combines guided lectures, demonstrations, and hands-on laboratory sessions to help participants understand how CRISPR-Cas9 experiments are planned, performed, analyzed, and interpreted.
By the end of the course, participants will have a strong foundation in CRISPR-Cas9 workflows and will be able to understand the key experimental steps required for successful genome editing projects.
Learning Objectives
By the end of this workshop, participants will be able to:
- Understand the basic principles and mechanism of CRISPR-Cas9 genome editing
- Explain the role of Cas9, gRNA, PAM sequence, and target DNA
- Understand how CRISPR-Cas9 experiments are designed
- Select suitable target regions and understand basic gRNA design principles
- Apply BSL-2 laboratory safety guidelines in genome editing-related workflows
- Understand reagent preparation steps for CRISPR-Cas9 experiments
- Become familiar with transfection methods and their applications
- Perform or observe key molecular biology techniques including DNA extraction and PCR
- Understand the role of gel electrophoresis in CRISPR workflow analysis
- Interpret basic experimental outcomes from CRISPR-related assays
- Recognize common technical challenges in CRISPR-Cas9 experiments
- Apply troubleshooting strategies for experimental planning and analysis
Target Audience
This course is ideal for:
- Master’s and PhD students
- Research assistants and laboratory technicians
- Biotechnology and biomedical science students
- Researchers new to genome editing technologies
- Scientists working in molecular biology, cell biology, genetics, or biotechnology
- Professionals who want to gain practical insight into CRISPR-Cas9 workflows
- Participants preparing for laboratory work involving gene editing, PCR, DNA analysis, or transfection methods
Course Program
Day 1: Introduction to CRISPR-Cas9 & Design Principles
| Time | Activity | Format |
|---|---|---|
| 09:00 – 09:30 | Introduction & Course Overview | Lecture |
| 09:30 – 11:00 | Introduction to BSL-2 Guidelines and Laboratory Safety | Lecture |
| 11:00 – 11:15 | Coffee Break | |
| 11:15 – 12:30 | CRISPR-Cas9 Theory: Background, Mechanism and Applications | Lecture |
| 12:30 – 13:30 | Lunch Break | |
| 13:30 – 15:00 | CRISPR-Cas9 Target Selection and Experimental Design Principles | Lecture / Workshop |
| 15:00 – 15:15 | Coffee Break | |
| 15:15 – 16:30 | Introduction to gRNA Design Tools and Target Evaluation | Workshop |
| 16:30 – 17:00 | Group Discussion, Questions and Preparation for Day 2 | Discussion & Q&A |
Day 2: Reagent Preparation & Transfection Methods
| Time | Activity | Format |
|---|---|---|
| 09:00 – 09:20 | Welcome, Recap of Day 1 and Questions | Q&A |
| 09:20 – 10:30 | CRISPR-Cas9 Reagent Preparation: Key Components and Workflow Planning | Lecture / Demonstration |
| 10:30 – 10:45 | Coffee Break | |
| 10:45 – 12:00 | CRISPR-Cas9 Reagent Preparation Practice or Demonstration | Laboratory |
| 12:00 – 13:00 | Lunch Break | |
| 13:00 – 14:00 | Introduction to Transfection Methods and Experimental Considerations | Lecture |
| 14:00 – 15:30 | CRISPR-Cas9 Transfection Workflow: Setup and Practical Application | Laboratory |
| 15:30 – 15:45 | Coffee Break | |
| 15:45 – 16:30 | Factors Affecting Transfection Efficiency and Cell Viability | Discussion |
| 16:30 – 17:00 | Documentation, Troubleshooting Discussion and Preparation for Day 3 | Discussion & Q&A |
Day 3: DNA Handling, PCR & Gel Electrophoresis
| Time | Activity | Format |
|---|---|---|
| 09:00 – 09:20 | Welcome, Recap of Day 2 and Questions | Q&A |
| 09:20 – 10:30 | DNA Extraction and PCR: Theory and Workflow Overview | Lecture |
| 10:30 – 11:00 | Coffee Break | |
| 11:00 – 12:00 | DNA Extraction from Cell Samples | Laboratory |
| 12:00 – 13:00 | Lunch Break | |
| 13:00 – 14:30 | PCR Setup, Amplification Strategy and Sample Preparation | Laboratory |
| 14:30 – 15:15 | Introduction to Agarose Gel Electrophoresis | Lecture / Demonstration |
| 15:15 – 15:30 | Coffee Break | |
| 15:30 – 16:30 | Gel Loading, Electrophoresis and Visualization of PCR Products | Laboratory |
| 16:30 – 17:00 | Result Documentation and Discussion | Discussion & Q&A |
Day 4: Data Analysis, Troubleshooting & Scientific Interpretation
| Time | Activity | Format |
|---|---|---|
| 09:00 – 09:20 | Welcome, Recap of Day 3 and Questions | Q&A |
| 09:20 – 10:30 | Introduction to CRISPR-Cas9 Outcome Analysis | Lecture |
| 10:30 – 10:45 | Coffee Break | |
| 10:45 – 12:00 | Data Interpretation: PCR and Gel Electrophoresis Results | Workshop |
| 12:00 – 13:00 | Lunch Break | |
| 13:00 – 14:00 | Common Challenges in CRISPR-Cas9 Experiments | Lecture & Discussion |
| 14:00 – 15:00 | Troubleshooting Case Studies: Design, Transfection, PCR and Analysis | Workshop |
| 15:00 – 15:30 | Group Discussion and Experimental Planning Reflection | Discussion |
| 15:30 – 16:00 | Final Quiz, Feedback Session and Certificate Information | Q&A |
What Participants Will Gain
Participants will gain:
- Practical understanding of CRISPR-Cas9 genome editing workflows
- Knowledge of CRISPR-Cas9 theory, mechanism, and experimental design
- Experience with target selection and gRNA design principles
- Understanding of BSL-2 safety and sterile workflow requirements
- Insight into CRISPR-Cas9 reagent preparation
- Practical experience with transfection workflow concepts
- Hands-on or demonstration-based experience with DNA extraction
- Practical knowledge of PCR setup and amplification
- Understanding of gel electrophoresis and result visualization
- Basic skills in CRISPR-related data interpretation
- Troubleshooting strategies for common CRISPR-Cas9 experimental issues
- Preparation for future work in genome editing, molecular biology, and biotechnology research
What participants say
I participated in a 4-day hands-on CRISPR course, which was very well organized both in terms of timing and content. Before the training, participants received materials to get familiar with the topic, which was very helpful. Each module included a theoretical introduction, followed by a hands-on demonstration and a practical session to apply what we had learned.
The instructors were very friendly, supportive, and took the time to address individual questions and challenges. It’s also great that participants can reach out to XTechAcademy even after the course for further questions or support.
The laboratory was modern and well-equipped, providing an excellent environment for learning. The building also offered a comfortable lounge area where participants could freely enjoy drinks, fruit, and even prepare something to eat in between sessions.
Overall, it was a great learning experience and an excellent opportunity to deepen practical skills in CRISPR technology.
Reviews are written by participants of our courses and are shown here unedited.
