4-Day Hands-On CRISPR-Cas9 Training Program

🔍 Overview

This 4-day hands-on training course introduces the principles and laboratory workflow of CRISPR-Cas9 genome editing, from gRNA design to data analysis. Participants will explore target selection, reagent preparation, and transfection methods in a guided lab environment.

The course also includes core molecular biology techniques such as DNA extraction, PCR amplification, and gel electrophoresis. Designed for Springer Campus students, this course ensures practical experience with genome editing and strengthens interpretation and troubleshooting skills essential for research success.

The program concludes with data analysis, discussion, and a short quiz to reinforce core concepts and assess learning outcomes.

📘 Modules

• CRISPR-Cas9 Theory, Mechanism & Design
• BSL-2 Lab Safety & Sterile Workflow
• Reagent Preparation & Transfection Methods
• DNA Extraction, PCR & Gel Electrophoresis
• Editing Outcome Analysis & Troubleshooting

📘Time Plan & Schedule

Day 1 – Introduction & CRISPR-Cas9 Design Principles

09:00 – 11:00 Introduction to BSL-2 Guidelines

11:00 – 11:15 Break

11:15 – 12:30 CRISPR-Cas9 Theory – Background & Mechanism

12:30 – 13:30 Lunch Break

13:30 – 17:00 CRISPR-Cas9 Target Selection & Design Tools

Day 2 – Reagent Preparation & Transfection

09:00 – 12:00 CRISPR-Cas9 Reagent Preparation (Demo & Hands-on)

12:00 – 13:00 Lunch Break

13:00 – 17:00 CRISPR-Cas9 Transfection Methods & Application

Day 3 – DNA Handling & PCR

09:00 – 10:30 DNA Extraction & PCR – Theory

10:30 – 11:00 Break

11:00 – 12:00 DNA Extraction – Hands-On

12:00 – 13:00 Lunch Break

13:00 – 17:00 PCR Setup, Amplification & Gel Electrophoresis

Day 4 – Data Analysis & Troubleshooting

09:00 – 12:00 Data Interpretation & Troubleshooting

12:00 – 13:00 Lunch Break

13:00 – 15:00 Data Interpretation & Troubleshooting