3-Day Intensive Cell Culture Training

🔍 Overview

This 3-day intensive workshop offers essential hands-on training in mammalian cell culture techniques, covering both adherent and non-adherent cells. Participants will be guided through the full workflow from BSL-2 safety standards and cell seeding to viability testing and troubleshooting.

Designed for Springer Campus students, this program bridges theory and lab practice while ensuring participants gain confidence in sterile technique, assay implementation, and data interpretation.

The training concludes with an interactive quiz and wrap-up discussion to reinforce learning outcomes and prepare participants for independent lab work.

📘 Modules

• BSL-2 Guidelines & Aseptic Technique
• Adherent vs. Suspension Cell Handling
• Thawing, Seeding & Passaging
• Cell Viability Assays (Trypan Blue, MTT)
• Freezing & Storage Methods
• Growth Curve Analysis & Troubleshooting

📘Time Plan & Schedule

Day 1 – BSL-2 & Seeding Fundamentals (Adherent & Non-Adherent Cells)

09:00 – 11:00 Introduction to BSL-2 Guidelines

11:00 – 11:15 Break

11:15 – 12:30 Cell Culture Theory (Adherent vs. Suspension)

12:30 – 13:30 Lunch Break

13:30 – 17:00 Thawing & Seeding (Both Cell Types)

Day 2 – Passaging & Viability Testing (Adherent & Non-Adherent Cells)

09:00 – 11:00 Passaging Techniques

11:00 – 11:15 Break

11:15 – 12:30 Cell Counting: Manual & Automated

12:30 – 13:30 Lunch Break

13:30 – 17:00 Trypan Blue / MTT Assay for Viability

Day 3 – Hood Mastery, Troubleshooting & Interpretation

09:00 – 11:00 Hood Technique Refinement

11:00 – 11:15 Break

11:15 – 12:30 Data Interpretation Workshop

12:30 – 13:30 Lunch Break

13:30 – 17:00 Troubleshooting Common Issues + Final Quiz