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Western Blot & Protein Quantification

Course Details

  • Location: XTech Academy, Heidelberg
  • Duration: 4 days
  • Format: Hands-on training course
  • Level: Beginner to intermediate

Workshop Schedule:

Additional information

🔍 Overview

This practical 4-day course offers in-depth training in protein analysis using SDS-PAGE and Western blotting, with a focus on quantitative interpretation through densitometry. Participants will learn the complete workflow from protein extraction and quantification to electrophoresis, membrane transfer, antibody-based detection, and data analysis.

The course emphasizes precision, reproducibility, and troubleshooting in blot development, antibody selection, and signal detection methods (chemiluminescence and fluorescence). In addition to lab techniques, participants will explore key concepts in protein normalization and quantification, equipping them with the skills needed for reliable protein expression studies.

Whether you’re entering molecular biology, cancer research, or biotechnology, this course will build your competence in one of the most widely used techniques in protein science.

 

📘 Modules

  • Protein Extraction & SDS-PAGE
  • Membrane Blotting & Transfer Techniques
  • Antibody Incubation & Signal Detection
  • Quantification, Normalization & Densitometric Analysis

 

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