Single-Cell RNA Sequencing (scRNA-seq) Essentials

🔍 Overview

This focused 3-day course introduces participants to the fundamental principles and workflows of single-cell RNA sequencing (scRNA-seq), with a strong emphasis on experimental planning and hands-on lab practice.

Participants will learn how to prepare high-quality single-cell suspensions, assess cell viability, and perform library generation using the 10x Genomics Chromium platform. The course also provides a beginner-friendly introduction to data quality metrics and the basics of downstream analysis, ensuring a well-rounded understanding of the complete scRNA-seq pipeline.

Ideal for researchers beginning their journey in single-cell transcriptomics, this course bridges the gap between wet-lab execution and data-driven interpretation.

📘 Modules

• Single-Cell Suspension Preparation & QC
• Genomics Library Generation Workflow
• Sample Barcoding & GEM Formation
• Introduction to Data Quality Metrics & Basic Analysis