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RT-qPCR & Gene Expression Analysis

Course Details

  • Location: XTech Academy, Heidelberg
  • Duration: 3 days
  • Format: Hands-on training course
  • Level: Beginner to intermediate

Workshop Schedule:

Additional information

🔍 Overview

This practical training course provides participants with a comprehensive introduction to the workflow and critical considerations for quantitative real-time PCR (RT-qPCR) in gene expression analysis. Over three intensive days, participants will gain hands-on experience in all major steps from RNA extraction and quality assessment to cDNA synthesis, qPCR setup, and data interpretation.

Participants will be trained in best practices, proper experimental design, and troubleshooting strategies. In addition to technical skills, the course emphasizes quantitative analysis, normalization techniques, and statistical validation, ensuring that attendees leave with both practical competence and conceptual understanding.

Whether you are transitioning into molecular biology research or want to strengthen your gene expression skills for diagnostics or biotechnology applications, this course equips you with essential knowledge and experience.

 

📘 Modules

  • RNA Extraction & Quality Assessment
  • cDNA Synthesis & qPCR Setup
  • Real-Time qPCR & Data Analysis
  • Advanced Troubleshooting & MIQE Guidelines

 

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