Molecular Cloning & Plasmid Design

🔍 Overview

This intensive hands-on course provides a solid foundation in molecular cloning techniques and plasmid construct design for research and biotech applications. Participants will learn the essential steps of classical cloning restriction digestion, ligation, transformation and gain experience with both theoretical planning and practical execution of cloning experiments.

Emphasis is placed on understanding vector architecture, insert selection, enzyme compatibility, and troubleshooting failed experiments. By the end of the course, participants will have designed and cloned their own plasmid construct using standard cloning methods and gained the confidence to apply these techniques in their own projects.

This workshop is ideal for anyone seeking practical lab skills in DNA manipulation, whether in academic labs, biotech startups, or synthetic biology projects.

📘 Modules

• Plasmid Structure & Cloning Strategy
• Restriction Enzyme Digestion & Gel Electrophoresis
• DNA Ligation & Vector-Inserts Assembly
• Bacterial Transformation & Clone Screening