CRISPR + Flow Cytometry Workshops

🔍 Overview

This dual-module program offers two in-depth, hands-on workshops focused on cutting-edge genome engineering and cellular analysis tools. Participants may attend either workshop independently or combine both for a comprehensive training experience.

Module 1: CRISPR Genome Engineering
Gain hands-on experience with CRISPR-Cas9 for gene editing in mammalian cells. The course covers guide RNA design, delivery methods, optimization strategies, and validation techniques. Participants will perform editing experiments and learn troubleshooting and analysis methods using T7E1 assay and sequencing.

Module 2: Flow Cytometry & Cell Sorting
Explore the power of flow cytometry for immunophenotyping, viability analysis, and cell sorting. Participants will gain practical experience with multicolor panel design, antibody staining, compensation, and FACS-based sorting of CRISPR-edited or transfected cells.

This program is ideal for researchers aiming to implement genome editing and advanced phenotypic screening in their own projects.

📘 Modules

• CRISPR Design, Delivery & Editing Strategies
• Genotyping & Editing Validation Techniques
• Flow Cytometry Panel Design & Staining
• Fluorescence-Activated Cell Sorting (FACS) & Data Interpretation