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Intensive Cell Culture Training | 3-Day Hands-On

Intensive Cell Culture Training

Hands-on

Course Details

Overview

This intensive 3-day hands-on cell culture workshop is designed for students, researchers, laboratory technicians, and professionals who want to gain practical experience in mammalian cell culture techniques.

The course covers both adherent and non-adherent cell culture systems, guiding participants through the complete workflow from BSL-2 safety principles, aseptic technique, cell thawing, seeding, passaging, counting, viability testing, and troubleshooting.

Participants will work directly in the laboratory and practice essential techniques required for independent cell culture work. The workshop combines theoretical background with practical training to help participants build confidence in sterile handling, experimental planning, and data interpretation.

By the end of the course, participants will have a strong foundation in routine mammalian cell culture and will be able to perform basic cell culture workflows safely and independently.

Learning Objectives

By the end of this workshop, participants will be able to:

  • Understand basic principles of mammalian cell culture
  • Apply BSL-2 laboratory safety guidelines
  • Perform aseptic work under a biosafety cabinet
  • Handle both adherent and non-adherent cells
  • Thaw, seed, and maintain mammalian cells
  • Perform cell passaging using appropriate techniques
  • Count cells manually and understand automated cell counting principles
  • Assess cell viability using Trypan Blue and MTT-based methods
  • Recognize common contamination risks and technical problems
  • Troubleshoot basic cell culture issues
  • Interpret simple cell culture results and document experiments properly

Target Audience

This course is ideal for:

  • Master’s and PhD students
  • Research assistants and laboratory technicians
  • Early-career researchers
  • Biotechnology and biomedical science students
  • Professionals entering cell culture-based laboratory work
  • Scientists who want to refresh or strengthen their basic cell culture skills
  • Participants preparing for laboratory work in academia, biotech, or pharmaceutical research

Course Program

Day 1: BSL-2 Safety, Aseptic Technique & Seeding Fundamentals

Time Activity Format
09:00 – 09:30 Introduction & Course Overview Lecture
09:30 – 10:30 Introduction to BSL-2 Guidelines and Laboratory Safety Lecture
10:30 – 10:45 Coffee Break
10:45 – 12:00 Principles of Mammalian Cell Culture: Adherent vs. Non-Adherent Cells Lecture
12:00 – 12:30 Introduction to Aseptic Technique and Biosafety Cabinet Work Demonstration
12:30 – 13:30 Lunch Break
13:30 – 15:00 Cell Thawing and Recovery: Preparing Cells for Culture Laboratory
15:00 – 15:15 Coffee Break
15:15 – 16:30 Cell Seeding Practice: Adherent and Non-Adherent Cell Systems Laboratory
16:30 – 17:00 Experimental Documentation, Discussion & Preparation for Day 2 Discussion & Q&A

Day 2: Passaging, Cell Counting & Viability Testing

Time Activity Format
09:00 – 09:20 Welcome, Recap of Day 1 and Questions Q&A
09:20 – 10:30 Principles of Cell Passaging and Culture Maintenance Lecture
10:30 – 10:45 Coffee Break
10:45 – 12:00 Passaging of Adherent Cells: Trypsinization and Re-Seeding Laboratory
12:00 – 12:30 Handling and Maintenance of Non-Adherent Cells Laboratory
12:30 – 13:30 Lunch Break
13:30 – 14:30 Manual Cell Counting Using Hemocytometer Laboratory
14:30 – 15:15 Introduction to Automated Cell Counting and Comparison of Methods Demonstration
15:15 – 15:30 Coffee Break
15:30 – 16:30 Cell Viability Assessment Using Trypan Blue Laboratory
16:30 – 17:00 Data Recording, Calculation Practice & Troubleshooting Discussion Discussion & Q&A

Day 3: Hood Mastery, Viability Assays, Troubleshooting & Interpretation

Time Activity Format
09:00 – 09:20 Welcome, Recap of Day 2 and Questions Q&A
09:20 – 10:30 Hood Technique Refinement: Best Practices for Sterile Work Laboratory
10:30 – 10:45 Coffee Break
10:45 – 12:00 Common Cell Culture Problems: Contamination, Poor Growth, Cell Death and Handling Errors Lecture & Discussion
12:00 – 12:30 Introduction to MTT Assay and Cell Viability Readouts Lecture
12:30 – 13:30 Lunch Break
13:30 – 14:45 MTT Assay Workflow or Demonstration-Based Viability Analysis Laboratory / Demonstration
14:45 – 15:30 Data Interpretation Workshop: Understanding Cell Growth and Viability Results Workshop
15:30 – 15:45 Coffee Break
15:45 – 16:30 Troubleshooting Case Studies and Group Discussion Discussion
16:30 – 17:00 Final Quiz, Feedback Session and Certificate Information Q&A

What Participants Will Gain

Participants will gain:

  • Hands-on experience in mammalian cell culture workflows
  • Practical laboratory skills in sterile and aseptic handling
  • Confidence working under a biosafety cabinet
  • Experience with adherent and non-adherent cell systems
  • Practical knowledge of thawing, seeding, passaging, and maintaining cells
  • Experience with manual cell counting and viability assessment
  • Understanding of Trypan Blue and MTT-based viability assays
  • Troubleshooting strategies for common cell culture problems
  • Better understanding of cell culture documentation and data interpretation
  • Preparation for independent laboratory work in academic or industrial settings

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